Chronotropic action of immobilized subtilisins during the perfusion of an isolated rat heart
نویسندگان
چکیده
The pharmacological experiments on isolated organs (ex vivo) are the preferred method for assessing primary pharmacodynamics of studied drugs, since this is completely excluded systemic influence neurohumoral regulation. In last decade, a new group thrombolytic drugs based immobilized subtilisins has been formed. At stage registrational preclinical and clinical studies, their pleiotropic effects have not studied. Meanwhile, there reason to consider that activity in bloodstream limited action, but may be extended effect cardiovascular system. aim study was investigate chronotropic an heart during its perfusion with solutions at different concentrations. Material methods. rat model according Langendorff used study. experiment included 50 Wistar rats, which were divided into 5 groups: hearts perfused only Krebs – Henseleit solution (control) or 4 concentrations (170, 340, 510 и 1020 U/l). Results discussion. negative effect. onset depends drug concentration solution: higher concentration, earlier From 10 minutes perfusion, observed using any dose. duration increase manifested up 10–20 minutes, depending solution. After 20 achieved remains plateau level 40 minutes. Conclusion. independent rate.
منابع مشابه
the effect of e-64 on the developmental competence of sheep cocs during in vitro maturation
in the present study, the effect of e-64 at different concentrations (0.5, 1, 5 and 10 µm) added to (1) the ivm medium on oocyte nuclear maturation and developmental competence of ovine oocytes, and (2) to the ivc medium on embryonic development of ovine embryos were investigated.
Effects of Postconditioning, Preconditioning and Perfusion of L-carnitine During Whole Period of Ischemia/ Reperfusion on Cardiac Hemodynamic Functions and Myocardial Infarction Size in Isolated Rat Heart
متن کامل
New manifestations of electrophysiological remodeling of heart during experimental model of atrial fibrillation in cirrhotic rat isolated heart
Introduction: The present study is aimed to evaluate electrophysiological remodeling of atrioventricular (AV) node and ventricular conduction during experimental atrial fibrillation (AF) model in isolated heart of cirrhotic rats. Methods: Cirrhosis-induced electrophysiological remodeling was evaluated in 24 isolated retrogradely perfused rat hearts in 2 groups (control and cirrhotic). Cirrho...
متن کاملan investigation of the types of text reduction in subtitling: a case study of the persian film gilaneh with english subtitles
چکیده ندارد.
15 صفحه اولEffects of Low Level of Lead Exposure on Responsiveness of The Rat Isolated Heart to Adrenergics
Background: There are controversial reports about the exact mechanisms of lead-induced hypertension, but many factors such as alteration in the responsiveness of cardiovascular system to endogenous substances including catecholamines could be one of the mechanisms involved. In present study, the effect of exposure to 100 ppm lead acetate by drinking water (in the periods of 4, 8 and 12 weeks)...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Sibirskij nau?nyj medicinskij žurnal
سال: 2022
ISSN: ['2410-2512', '2410-2520']
DOI: https://doi.org/10.18699/ssmj20220505